Journal: International Journal of Molecular Sciences

Article Title: Targeting PKCθ Promotes Satellite Cell Self-Renewal

doi: 10.3390/ijms21072419

Figure Lengend Snippet: Lack of PKCθ stimulates symmetric self-renewal by regulating Pard3 polarization. ( A ): Representative pictures of single myofibers isolated from EDL muscles of WT and PKCθ-/- mice, after 48h in culture. Myofibers were stained for Pax7 (red) and MyoD (green), nuclei were counterstained with Topro3. ( B ): Quantification of symmetric division events. ( C ): Quantification of Pax7 + /MyoD − cell doublets, and ( D ): quantification of total Pax7 + /MyoD − cells in WT and PKCθ-/- single myofibers. ( E ): Representative pictures of single myofibers isolated from EDL muscles of WT and PKCθ-/- mice, after 36h in culture. Myofibers were stained for Pax7 (red) and Pard3 (green), nuclei were counterstained with Topro3. ( F ): Percentage ofSCs showing symmetric, low or asymmetric Pard3 distribution in WT and PKCθ-/- myofibers. ( G ): Percentage of SCs showing symmetric and asymmetric Pard3 distribution (WT, n = 3 mice, PKCθ-/-, n = 3 mice, n > 20 myofibers analyzed per mouse). Error bars represent mean ± sem, * p < 0.05 calculated by Student’s t -test.

Article Snippet: Primary antibodies mouse anti Pax7, rabbit anti-MyoD (1:50 Santa Cruz C20: sc-304, Dallas, TX, USA), and rabbit anti Pard3 (07-330 Merck Millipore, Burlington, MA, USA) were incubated O/N at 4 °C.

Techniques: Isolation, Staining